Description
uFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme uBA5 and E2-like conjugating enzyme uFC1 in a manner analogous to ubiquitylation. It localizes primarily to the nucleus, but is also present in diffuse amounts in the cytoplasm. This protein is expressed in a variety of tissues, including kidney, brain, heart, liver and lung. Recombinant human uFM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques